Exploring New Conservation Methods: Isolation and Characterization of Algicidal Bacteria from Ornamental Fountains in the Alhambra and Generalife (Granada, Spain)

Researchers have identified a highly effective algicidal bacterium, Stenotrophomonas maltophilia strain LIG25, capable of inhibiting over 98% of algal growth in ornamental fountains at the Alhambra and Generalife (Granada, Spain). This discovery offers a promising eco-friendly alternative to conventional chemical biocides for conserving cultural heritage sites vulnerable to biodeterioration. The study employed 16S rRNA gene sequencing to characterize bacterial communities from eight fountain samples, revealing 18 genera with reported algicidal activity. Functional screening assays using filtered fountain waters against Chlorella vulgaris demonstrated intrinsic inhibitory capacity, with the most active sample selected for further isolation and validation through co-culture assays, cell density measurements, and PAM fluorometry. Despite detecting a diverse microbiome, no significant link was found between taxonomic composition and biofilm development, underscoring the complexity of microbial interactions in these environments.

Calvo-Bayo, I., Fillet, S., Cuzman, O. A., Cuberos-Cáceres, L., González-del-Valle, M., Bolívar-Galiano, F., & Romero-Noguera, J. (2026). Exploring New Conservation Methods: Isolation and Characterization of Algicidal Bacteria from Ornamental Fountains in the Alhambra and Generalife (Granada, Spain). Conservation, 6(2), 70. https://doi.org/10.3390/conservation6020070