Comparación de técnicas para la extracción de bacterias coliformes del sedimento del lago de Xochimilco, México

Researchers found that the use of ampicillin significantly increased the concentration of total coliform bacteria extracted from Xochimilco Lake sediment compared to traditional methods like manual shaking, homogenization, and ultrasonication. This suggests that ampicillin could be an effective technique for extracting coliforms, though its potential impact on bacterial sensitivity must be considered. The study compared multiple extraction techniques, including the use of enzymes (pancreatine) and surfactants, but only ampicillin showed a statistically significant difference (p = 0.03). Samples were collected from Xochimilco Lake in Mexico City, with the most probable number of coliform bacteria determined after applying each technique.

Fernández-Rendón, C. L., & Barrera-Escorcia, G. (2013). Comparación de técnicas para la extracción de bacterias coliformes del sedimento del lago de Xochimilco, México. Revista Argentina de Microbiología, 45(3), 180–184. https://doi.org/10.1016/s0325-7541(13)70022-1