A PCR-RFLP method to detect hybridization between the invasive Eurasian watermilfoil (Myriophyllum spicatum) and the native northern watermilfoil (Myriophyllum sibiricum), and its application in Ontario lakes

Researchers have identified hybridization between the invasive Eurasian watermilfoil (Myriophyllum spicatum) and the native northern watermilfoil (Myriophyllum sibiricum), with four hybrid samples detected in three adjacent lakes linked to the Rideau Canal Waterway. This discovery, confirmed using a novel PCR-RFLP screening method, addresses challenges in morphological identification due to overlapping traits. The study validated this approach against traditional sequencing, demonstrating its efficiency for large-scale sampling. Findings suggest the hybrid's potential invasiveness and distribution along connected waterways, offering insights for managing aquatic ecosystems. This work provides a critical tool for monitoring hybridization in Ontario lakes.

Grafe, S. F., Boutin, C., Pick, F. R., & Bull, R. D. (2015). A PCR-RFLP method to detect hybridization between the invasive Eurasian watermilfoil (Myriophyllum spicatum) and the native northern watermilfoil (Myriophyllum sibiricum), and its application in Ontario lakes. Botany, 93(2), 117–121. https://doi.org/10.1139/cjb-2014-0135