Gluconacetobacter tumulisoli sp. nov., Gluconacetobacter takamatsuzukensis sp. nov. and Gluconacetobacter aggeris sp. nov., isolated from Takamatsuzuka Tumulus samples before and during the dismantling work in 2007

Researchers have identified three novel species of bacteria, Gluconacetobacter tumulisoli, Gluconacetobacter takamatsuzukensis, and Gluconacetobacter aggeris, isolated from soil samples collected before and during the dismantling work on the Takamatsuzuka Tumulus in Japan's Asuka village. These discoveries were made using a polyphasic approach that included 16S rRNA gene sequence analysis, DNA-DNA hybridization tests, and characterization of their biochemical properties. The new species are distinct from known Gluconacetobacter strains, with Q-10 as the major ubiquinone system and C18:1ω7c as the predominant fatty acid. This study contributes to the understanding of microbial diversity in archaeological sites, particularly in UNESCO World Heritage Sites like the Ancient Capitals of Asuka and Fujiwara.

Nishijima, M., Tazato, N., Handa, Y., Tomita, J., Kigawa, R., Sano, C., & Sugiyama, J. (2013). Gluconacetobacter tumulisoli sp. nov., Gluconacetobacter takamatsuzukensis sp. nov. and Gluconacetobacter aggeris sp. nov., isolated from Takamatsuzuka Tumulus samples before and during the dismantling work in 2007. International Journal of Systematic and Evolutionary Microbiology, 63(Pt_11), 3981–3988. https://doi.org/10.1099/ijs.0.051292-0